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Antibody used in IHC on Human Muscle.
Antibody used in WB on Human Daudi at 0.2-1 ug/ml.
Antibody used in IHC on Human Muscle.

GTF2E1 Antibody

CATALOG NUMBER: 31-216

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2E1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
GTF2E1 antibody can be used for detection of GTF2E1 by ELISA at 1:1562500. GTF2E1 antibody can be used for detection of GTF2E1 by western blot at 0.2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Predicted Molecular Weight:
49 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store GTF2E1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
GTF2E1
Additional Names:
GTF2E1, FE, TF2E1, TFIIE-A
Protein Accession Number:
NP_005504
Protein Gi Number:
144922725
Ncbi Gene Id Number:
2960
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation
Background Reference 1:
Ohkuma, Y., et al., (1991) Nature 354 (6352), 398-401.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-216

List Size:
100 ul

List Price:

$519.00

Datasheet
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