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Western blot analysis in 293 cell line lysates (35ug/lane).
GTF2E1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in 293 cell line lysates (35ug/lane).

GTF2E1 Antibody

CATALOG NUMBER: 57-730

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This GTF2E1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-121 amino acids from the N-terminal region of human GTF2E1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
49 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GTF2E1
Additional Names:
General transcription factor IIE subunit 1, General transcription factor IIE 56 kDa subunit, Transcription initiation factor IIE subunit alpha, TFIIE-alpha, GTF2E1, TF2E1
Protein Accession Number:
P29083
Protein Gi Number:
116242812
Ncbi Gene Id Number:
2960
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Background Reference 1:
Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
Background Reference 2:
Okuda, M., et al. EMBO J. 27(7):1161-1171(2008)
Background Reference 3:
Di Lello, P., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):106-111(2008)
Background Reference 4:
Jawhari, A., et al. EMBO Rep. 7(5):500-505(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-730

List Size:
400 ul

List Price:

$495.00

Datasheet
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