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Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Antibody used in WB on Human 293T at 0.2-1 ug/ml.

GTF2E2 Antibody

CATALOG NUMBER: 25-409

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2E2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
GTF2E2 antibody can be used for detection of GTF2E2 by ELISA at 1:312500. GTF2E2 antibody can be used for detection of GTF2E2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
293T Cell Lysate
Predicted Molecular Weight:
33 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store GTF2E2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
GTF2E2
Additional Names:
GTF2E2, FE, TF2E2, TFIIE-B
Protein Accession Number:
NP_002086
Protein Gi Number:
4504195
Ncbi Gene Id Number:
2961
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GTF2E2 belongs to the TFIIE beta subunit family.It recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Background Reference 1:
Watanabe, T., (2003) Mol. Cell. Biol. 23 (8), 2914-2926.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-409

List Size:
100 ul

List Price:

$519.00

Datasheet
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