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45-716 staining (0.2ug/ml) of Human Placenta lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
45-716 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-716 staining (0.2ug/ml) of Human Placenta lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

GULP1 Antibody

CATALOG NUMBER: 45-716

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: EGTVFCLDPLDSRC
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35kDa band observed in Human Placenta lysates (calculated MW of 34.5kDa according to NP_057399.1). Recommended concentration: 0.2-0.6ug/ml.An additional band of unknown identity was also consistently observed at approx.46kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of cytoplasm, stronger in a selection of epithelial cells of distal convoluted tubules. Recommended concentration: 5-10 ug/ml.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GULP1
Additional Names:
CED-6, GULP1, CED-6 protein, GULP, engulfment adaptor PTB domain containing 1, CED6, FLJ31156, OTTHUMP00000205908, OTTHUMP00000205909, OTTHUMP00000205911, PTB domain adaptor protein CED-6, engulfment adapter protein
Protein Accession Number:
NP_057399.1
Protein Gi Number:
7705318
Ncbi Gene Id Number:
51454
Background Reference 1:
Su HP, Nakada-Tsukui K, Tosello-Trampont AC, Li Y, Bu G, Henson PM, Ravichandran KS. Interaction of CED-6/GULP, an adapter protein involved in engulfment ofapoptotic cells with CED-1 and CD91/low density lipoprotein receptor-relatedprotein (LRP). J Biol Chem. 2002 Apr 5;277(14):11772-9. Epub 2001 Nov 29.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-716

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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