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Western blot analysis of GZMB Antibody in HL-60 cell line lysates (35ug/lane)
GZMB Antibody IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of GZMB using rabbit polyclonal GZMB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.
Western blot analysis of GZMB Antibody in HL-60 cell line lysates (35ug/lane)

GZMB Antibody

CATALOG NUMBER: 62-336

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GZMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-203 amino acids from the C-terminal region of human GZMB.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GZMB
Additional Names:
Granzyme B, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E, GZMB, CGL1, CSPB, CTLA1, GRB
Protein Accession Number:
P10144
Protein Gi Number:
317373361
Ncbi Gene Id Number:
3002
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
Background Reference 1:
Hagn,M., J. Immunol. 183 (3), 1838-1845 (2009)
Background Reference 2:
Gaafar,A., Exp. Hematol. 37 (7), 838-848 (2009)
Background Reference 3:
Girnita,D.M., Transplantation 87 (12), 1801-1806 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-336

List Size:
400 ul

List Price:

$495.00

Datasheet
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