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DBA/2 mouse splenocytes were stained with Mouse Anti-Mouse H-2Dd-PE (Cat. No. 99-083).
DBA/2 mouse splenocytes were stained with Mouse Anti-Mouse H-2Dd-PE (Cat. No. 99-083).

H-2Dd Antibody [34-5-8S] (PE)

CATALOG NUMBER: 99-083

Clonality:
Monoclonal
Tested Applications:
Flow, IHC-Fr, IHC-P, IP
Host Species:
Mouse
Species Reactivity:
Mouse
Conjugate:
PE
Specifications
Host Species:
Mouse
Species Reactivity:
Mouse
Immunogen:
BDF1 mouse splenocytes
Conjugate:
PE
Tested Applications:
Flow, IHC-Fr, IHC-P, IP
Application Note:
H-2Dd Antibody [34-5-8S] for use in flow cytometry assays.
Specificity:
H-2Dd
Clonality:
Monoclonal
Isotype:
IgG2a
physical-state:
Liquid
Buffer:
Supplied in PBS/NaN3 and a stabilizing agent
Concentration:
0.1 mg/mL
Storage Conditions:
Store vial at 2-8°C
Ncbi Official Symbol:
H2-D1
Additional Names:
MHC Class I
Protein Accession Number:
P01899
Ncbi Gene Id Number:
14964
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The monoclonal antibody 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with beta2-microglobulin. The antibody does not react with H-2Dd alpha chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

99-083

List Size:
0.1 mg

List Price:

$432.00

Datasheet
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