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<strong>Figure 1 Western Blot Validation with H1N1 NA Protein </strong><br> Loading: 50ng (Lane 1) and 100ng (Lane 2) H1N1 Neuraminidase recombinant protein. Antibodies: H1N1 NA antibody, 5245, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 ELISA Validation with H1N1 NA Protein </strong><br> Coating Antigen: H1N1 Neuraminidase recombinant protein, 2 μg/mL, incubated at 4 °C overnight. Detection Antibodies: H1N1 NA antibody, 5245, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit at 1:10,000, incubated at RT for 1 hr.
Figure 1 Western Blot Validation with H1N1 NA Protein
Loading: 50ng (Lane 1) and 100ng (Lane 2) H1N1 Neuraminidase recombinant protein. Antibodies: H1N1 NA antibody, 5245, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Trial Size

H1N1 Neuraminidase Antibody

CATALOG NUMBER: 5245

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Immunogen:
Neuraminidase antibody was raised against synthetic peptides from the seasonal influenza (H1N1) Neuraminidase protein and the novel swine influenza Neuraminidase protein.
The antibody recognizes both variants.
The immunogen is located within the first 50 amino acids of H1N1 Neuraminidase.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 2 μg/mL.
Neuraminidase antibody can be used for the detection of the Neuraminidase protein from the seasonal influenza A (H1N1) and the novel swine influenza in ELISA and WB. It will detect 2 ng of free peptide at 1 μg/mL.
Specificity:
This antibody will recognize the Neuraminidase from the both the seasonal influenza (H1N1) and swine-origin influenza virus (A/California/14/2009 (H1N1)).
Purification:
H1N1 Neuraminidase Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
H1N1 Neuraminidase Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
H1N1 Neuraminidase antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NA
Additional Names:
H1N1 Neuraminidase Antibody:
Protein Accession Number:
ACA33620;ACQ76308
Protein Gi Number:
168827347 / 229535824
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
H1N1 Neuraminidase Antibody: Influenza A virus is a major public health threat, killing more than 30, 000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic. Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible Neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin. The distinct antigenic properties of the new swine influenza virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited, although the age distribution of reported cases suggests some degree of protection in older age groups.
Background Reference 1:
Thompson WW, Shay DK, Weintraub, et al. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA2003; 289:179-186.
Background Reference 2:
Novel Swine-Origin Influenza A (H1N1) Virus Investigation Team, Dawood FS, Jain S, et al. Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N. Engl. J. Med.2009; 360:2605-15.
Background Reference 3:
Butler D. Swine flu goes global. Nature2009; 458:1082-3.
Background Reference 4:
Morens DM, Taubenberger JK, and Fauci AS. The Persistent Legacy of the 1918 Influenza Virus. N. Engl. J. Med.2009; Jun 29.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5245

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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