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Western blot analysis in K562 cell line lysates (35ug/lane).
H2AFX Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in K562 cell line lysates (35ug/lane).

H2AFX Antibody

CATALOG NUMBER: 57-219

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This H2AFX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human H2AFX.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
15 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
H2AFX
Additional Names:
Histone H2AX, H2a/x, Histone H2AX, H2AFX, H2AX
Protein Accession Number:
P16104
Protein Gi Number:
121992
Ncbi Gene Id Number:
3014
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsiblefor the nucleosome structure of the chromosomal fiber ineukaryotes. Two molecules of each of the four core histones (H2A,H2B, H3, and H4) form an octamer, around which approximately 146 bpof DNA is wrapped in repeating units, called nucleosomes. Thelinker histone, H1, interacts with linker DNA between nucleosomesand functions in the compaction of chromatin into higher orderstructures. This gene encodes a member of the histone H2A family,and generates two transcripts through the use of the conservedstem-loop termination motif, and the polyA addition motif.
Background Reference 1:
Roch-Lefevre, S., et al. Radiat. Res. 174(2):185-194(2010)
Background Reference 2:
Schmid, T.E., et al. Int. J. Radiat. Biol. 86(8):682-691(2010)
Background Reference 3:
Jiang, X., et al. FEBS Lett. 584(13):2926-2930(2010)
Background Reference 4:
Vasireddy, R.S., et al. Br. J. Cancer 102(10):1511-1518(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-219

List Size:
400 ul

List Price:

$495.00

Datasheet
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