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Western Blot at 1:1000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.

H2BFWT Antibody

CATALOG NUMBER: 58-632

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This H2BFWT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-88 amino acids from the Central region of human H2BFWT.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
20 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
H2BFWT
Additional Names:
Histone H2B type W-T, H2B histone family member W testis-specific, H2BFWT
Protein Accession Number:
Q7Z2G1
Protein Gi Number:
223590216
Ncbi Gene Id Number:
158983
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsiblefor the nucleosome structure of the chromosomal fiber ineukaryotes. Two molecules of each of the four core histones (H2A,H2B, H3, and H4) form an octamer, around which approximately 146 bpof DNA is wrapped in repeating units, called nucleosomes. Thelinker histone, H1, interacts with linker DNA between nucleosomesand functions in the compaction of chromatin into higher orderstructures. This gene encodes a member of the H2B histone familythat is specifically expressed in sperm nuclei. A polymorphism inthe 5' UTR of this gene is associated with male infertility.
Background Reference 1:
Lee, J., et al. J. Cell. Mol. Med. 13 (8B), 1942-1951 (2009) :
Background Reference 2:
Boulard, M., et al. Mol. Cell. Biol. 26(4):1518-1526(2006)
Background Reference 3:
Churikov, D., et al. Genomics 84(4):745-756(2004)
Background Reference 4:
Churikov, D., et al. Cytogenet. Genome Res. 105 (2-4), 203-214 (2004) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-632

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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