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<b> Immunofluorescence staining of fixed HeLa cells with anti-H3K27me3 antibody BT164 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of BT164 (24-812) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom 24-812- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Western Blot using anti-H3K27me3 antibody BT164 (</b> Nuclear lysate of HeLa(A) (0.0003ug/ml)- Jurkat(B) (0.0003ug/ml) and K562(C) (0.01ug/ml) (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of BT164 (24-812) before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
<b> Flow cytometry using the anti-H3K27me3 antibody BT164 ( </b> HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of BT164 (24-812- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of fixed HeLa cells with anti-H3K27me3 antibody BT164 ( Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of BT164 (24-812) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom 24-812- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

H3K27me3 Antibody [BT164], Rabbit IgG

CATALOG NUMBER: 24-812

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA, ICC, IF, IHC, IP, WB
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Mouse
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
BT164 is a mice-derrived lupus antibody that was established by selection on apoptotic chromatin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC, IP, WB
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Additional Names:
trimethyl-Histone H3 (Lys27), histone H3, H3K27me3, Histone H3 trimethylated at Lysine 27, nucleosome, H3K27ME3
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-812

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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