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<b> Flow cytometry using the anti-H4 K8Ac; K12Ac; K16Ac antibody KM-2 ( </b> HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of KM-2 (24-811- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Immunofluorescence staining of fixed HeLa cells with anti-H4 K8Ac; K12Ac; K16Ac antibody KM-2 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of KM-2 (24-811) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom 24-811- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the anti-H4 K8Ac; K12Ac; K16Ac antibody KM-2 ( HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of KM-2 (24-811- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

H4 K8Ac; K12Ac; K16Ac Antibody [KM-2], Rabbit IgG

CATALOG NUMBER: 24-811

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA, IF, IHC, WB
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Mouse
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
KM-2 antibody was derived from an MRL/lpr lupus mouse.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Additional Names:
histone 4, nucleosome, H4K8Ac, H4K12Ac, H4K16Ac, acetyl-Histone H4, Histone H4 acetylated at lysine, KM2
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-811

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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