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Western blot analysis of extracts of various cell lines, using H6PD Antibody (22-223) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using H6PD antibody (22-223) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using H6PD antibody (22-223). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using H6PD Antibody (22-223) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.

H6PD Antibody

CATALOG NUMBER: 22-223

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human H6PD (NP_004276.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
Mouse liver
Positive Control 2:
Mouse kidney
Predicted Molecular Weight:
Observed: 89kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
H6PD
Additional Names:
H6PD, DKFZp686A01246, G6PDH, GDH, MGC87643, CORTRD1
Ncbi Gene Id Number:
9563
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-223

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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