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Western blot analysis of HAAO in Mouse liver tissue lysate with HAAO antibody at 1 μg/mL.
Immunohistochemistry of HAAO in human liver tissue with HAAO antibody at 2.5 μg/mL.
Immunofluorescence of HAAO in Human Liver cells with HAAO antibody at 20 μg/mL.
Immunohistochemistry of HAAO in mouse colon tissue with HAAO antibody at 2 μg/mL.
Western blot analysis of HAAO in Mouse liver tissue lysate with HAAO antibody at 1 μg/mL.
Trial Size

HAAO Antibody

CATALOG NUMBER: 5251

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
HAAO antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human HAAO.
The immunogen is located within amino acids 30 - 80 of HAAO.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
HAAO antibody can be used for detection of HAAO by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Purification:
HAAO Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
HAAO Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
HAAO antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HAAO
Additional Names:
HAAO Antibody: HAO, 3-HAO, 3-hydroxyanthranilate 3, 4-dioxygenase, 3-hydroxyanthranilate oxygenase
Protein Accession Number:
NP_036337
Protein Gi Number:
188497625
Ncbi Gene Id Number:
23498
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HAAO Antibody: HAAO (3-Hydroxyanthranilate 3, 4-dioxygenase) is a monomeric cytosolic protein of the family of intramolecular dioxygenases containing non-heme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is present in low amounts in the central nervous system. This enzyme participates in tryptophan metabolism. It employs one cofactor, iron. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurological and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN. Furthermore, recent study shows that HAAO are excellent candidate biomarkers for detecting ovarian cancer.
Background Reference 1:
Decker RH, Kang HH, Leach FR, et al. Purification and properties of 3-hydroxyanthranilic acid oxidase. J. Biol. Chem.1961; 236:3076-82.
Background Reference 2:
Huang YW, Jansen RA, Fabbri E, et al. Identification of candidate epigenetic biomarkers for ovarian cancer detection. Oncol. Rep.2009; 22:853-61.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5251

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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