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Western Blot at 1:1000-1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: human liver lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: mouse liver lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
HABP2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000-1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: human liver lysate Lysates/proteins at 20 ug per lane.

HABP2 Antibody

CATALOG NUMBER: 62-002

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This HABP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-408 amino acids from the C-terminal region of human HABP2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000-1:2000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
63 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HABP2
Additional Names:
Hyaluronan-binding protein 2, 3421-, Factor VII-activating protease, Factor seven-activating protease, FSAP, Hepatocyte growth factor activator-like protein, Plasma hyaluronan-binding protein, Hyaluronan-binding protein 2 50 kDa heavy chain, Hyaluronan-binding protein 2 50 kDa heavy chain alternate form, Hyaluronan-binding protein 2 27 kDa light chain, Hyaluronan-binding protein 2 27 kDa light chain alternate form, HABP2, HGFAL, PHBP
Protein Accession Number:
Q14520
Protein Gi Number:
73919921
Ncbi Gene Id Number:
3026
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HABP2 is an extracellularserine protease that binds hyaluronic acid and is involved in celladhesion. The encoded protein is synthesized as a single chain, butthen undergoes an autoproteolytic event to form the functionalheterodimer. Further autoproteolysis leads to smaller, inactivepeptides. This protease is known to cleave urinary plasminogenactivator, coagulation factor VII, and the alpha and beta chains offibrinogen, but not prothrombin, plasminogen, or the gamma chain offibrinogen. Two transcript variants encoding different isoformshave been found for this gene.
Background Reference 1:
Choi-Miura, N.H., et al. Biol. Pharm. Bull. 24(2):140-143(2001)
Background Reference 2:
Sumiya, J., et al. J. Biochem. 122(5):983-990(1997)
Background Reference 3:
Choi-Miura, N.H., et al. J. Biochem. 119(6):1157-1165(1996)
Background Reference 4:
Gupta, S., et al. Eur. J. Cell Biol. 56(1):58-67(1991)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-002

List Size:
400 ul

List Price:

$495.00

Datasheet
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