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45-717 (0.02ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing HADH with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with 45-717 in the right panel (mock transfection in first and last lanes).
45-717 (0.02ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HADH Antibody

CATALOG NUMBER: 45-717

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-YERGDASKEDID
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 33kDa band observed in Human Heart, Muscle and Kidney lysates (calculated MW of 34.3kDa according to NP_005318.2). In transfected HEK293 transiently expressing HADH a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03ug/ml.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HADH
Additional Names:
HADH, HADHSC, hydroxyacyl-Coenzyme A dehydrogenase, HAD, HADH1, HHF4, M/SCHAD, MGC8392, SCHAD, L-3-hydroxyacyl-Coenzyme A dehydrogenase, L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain
Protein Accession Number:
NP_005318.2
Protein Gi Number:
94557308
Ncbi Gene Id Number:
3033
Background Reference 1:
Molven A, Matre GE, Duran M, Wanders RJ, Rishaug U, Njolstad PR, Jellum E, Sovik O. Familial hyperinsulinemic hypoglycemia caused by a defect in the SCHAD enzyme of mitochondrial fatty acid oxidation. Diabetes. 2004 Jan;53(1):221-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-717

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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