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45-068 staining (0.3ug/ml) of Human Brain lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
45-068 staining (0.3ug/ml) of Human Brain lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.

HADH2 Antibody

CATALOG NUMBER: 45-068

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: CIRLDGAIRMQP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 25kDa band observed in Human Brain lysates (calculated MW of 26.9kDa according to NP_004484). Recommended concentration: 0.3-2ug/ml. Primary incubation 1 hour at room temperature. A minor band of unknown identity was also consistently observed at appr 38kDa. This band was successfully blocked by incubation with the immunising peptide.
Specificity:
This antibody is expected to recognise both reported isoforms.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HSD17B10
Additional Names:
HSD17B10, HADH2, ABAD, ERAB, MHBD, 17b-HSD10, hydroxyacyl-Coenzyme A dehydrogenase, type II, type 10 17b-HSD, AB-binding alcohol dehydrogenase, type 10 17beta-hydroxysteroid dehydrogenase, hydroxysteroid (17-beta) dehydrogenase 10, RP3-339A18.2, 17b-HSD10, CAMR, DUPXp11.22, HCD2, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, 17-beta-hydroxysteroid dehydrogenase type 10, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, amyloid-beta binding polypeptide, amyloid-beta peptide binding alcohol dehydrogenase, mental retardation, X-linked, syndromic 10, mental retardation, X-linked, syndromic 11, short chain L-3-hydroxyacyl-CoA dehydrogenase type 2, short chain dehydrogenase/reductase family 5C, member 1, short chain type dehydrogenase/reductase XH98G2
Protein Accession Number:
NP_004484.1,NP_001032900.1
Protein Gi Number:
4758504
Ncbi Gene Id Number:
3028
Background Reference 1:
He XY, Schulz H, Yang SY. A human brain L-3-hydroxyacyl-coenzyme A dehydrogenase is identical to an amyloid beta-peptide-binding protein involved in Alzheimer's disease. J Biol Chem. 1998 Apr 24;273(17):10741-6.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-068

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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