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Western blot analysis of HADHB Antibody in Jurkat cell line lysates (35ug/lane)
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of HADHB Antibody in Jurkat cell line lysates (35ug/lane)

HADHB Antibody

CATALOG NUMBER: 64-152

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey
Immunogen:
This HADHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-342 amino acids from the C-terminal region of human HADHB.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
51 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HADHB
Additional Names:
Trifunctional enzyme subunit beta, mitochondrial, TP-beta, 3-ketoacyl-CoA thiolase, Acetyl-CoA acyltransferase, Beta-ketothiolase, HADHB
Protein Accession Number:
P55084
Protein Gi Number:
116241345
Ncbi Gene Id Number:
3032
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HADHB encodes the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. Mutations in this gene result in trifunctional protein deficiency. The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
Background Reference 1:
Purevsuren, J., et al. Mol. Genet. Metab. 98(4):372-377(2009)
Background Reference 2:
Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008)
Background Reference 3:
Wang, R., et al. Zhonghua Fu Chan Ke Za Zhi 41(10):672-675(2006)
Background Reference 4:
Hillier, L.W., et al. Nature 434(7034):724-731(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-152

List Size:
400 ul

List Price:

$495.00

Datasheet
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