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Western blot analysis of extracts of various cell lines, using HAL antibody (14-749) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using HAL antibody (14-749) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HAL antibody (14-749) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using HAL antibody (14-749) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HAL antibody (14-749) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using HAL Polyclonal Antibody (14-749) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HAL antibody (14-749) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

HAL Antibody

CATALOG NUMBER: 14-749

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HAL (NP_002099.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
Mouse liver
Positive Control 2:
Rat liver
Predicted Molecular Weight:
Observed: 73kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HAL
Additional Names:
HAL, HIS, HSTD, histidase
Ncbi Gene Id Number:
3034
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-749

List Size:
100 uL

List Price:

$436.00

Datasheet
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