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45-719 (3.8ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-719 (3.8ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

hALP Antibody

CATALOG NUMBER: 45-719

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KSDKKRKLEAKQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 26kDa band in Human Brain (Cerebellum, Frontal Cortex and Hippocampus) lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116kDa according to NP_078938.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Colon shows nuclear staining in absorptive cells. Recommended concentration, 3-6ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NAT10
Additional Names:
NAT10, N-acetyltransferase 10, ALP, DKFZp434C116, FLJ10774, FLJ12179, FLJ23850, KIAA1709, hALP , N-acetyltransferase-like, N-acetyltransferase-like protein
Protein Accession Number:
NP_078938.1
Protein Gi Number:
13399322
Ncbi Gene Id Number:
55226
Background Reference 1:
Yin J, Ma Z, Selliah N, Shivers DK, Cron RQ, Finkel TH. Effective gene suppression using small interfering RNA in hard-to-transfect human T cells. J Immunol Methods. 2006 May 30;312(1-2):1-11. Epub 2006 Mar 24.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-719

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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