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Fluorescent confocal image of Hela cell stained with HAND2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).HAND2 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in K562 cell line lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with HAND2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).HAND2 immunoreactivity is localized to Nucleus significantly.

HAND2 Antibody

CATALOG NUMBER: 58-872

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Rat, Xenopus
Immunogen:
This HAND2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the Central region of human HAND2.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HAND2
Additional Names:
Heart- and neural crest derivatives-expressed protein 2, Class A basic helix-loop-helix protein 26, bHLHa26, Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2, dHAND, HAND2, BHLHA26, DHAND
Protein Accession Number:
P61296
Protein Gi Number:
47117699
Ncbi Gene Id Number:
9464
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the basichelix-loop-helix family of transcription factors. This gene productis one of two closely related family members, the HAND proteins,which are asymmetrically expressed in the developing ventricularchambers and play an essential role in cardiac morphogenesis.Working in a complementary fashion, they function in the formationof the right ventricle and aortic arch arteries, implicating themas mediators of congenital heart disease. In addition, thistranscription factor plays an important role in limb and branchialarch development.
Background Reference 1:
Shen, L., et al. Chin. Med. J. 123(13):1623-1627(2010)
Background Reference 2:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
Background Reference 3:
Voth, H., et al. BMC Mol. Biol. 10, 28 (2009) :
Background Reference 4:
Morikawa, Y., et al. Circ. Res. 103(12):1422-1429(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-872

List Size:
400 ul

List Price:

$495.00

Datasheet
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