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45-721 (0.1ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-721 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-721 (0.1ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

HAP1 Antibody

CATALOG NUMBER: 45-721

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RYDFRYSEDREQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in Human Brain (Frontal Cortex and Hippocampus) lysates (calculated MW of 69.6kDa according to NP_817084.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_003940.1, NP_817084.1)
Positive Control 1:
Cat. No. XBL-10110 - Human Hippocampus Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HAP1
Additional Names:
HAP1, huntingtin-associated protein 1 (neuroan 1), HAP2, HIP5, HLP, hHLP1, huntingtin-associated protein 1, huntingtin-associated protein 2, neuroan 1
Protein Accession Number:
NP_003940.2,NP_817084.1
Protein Gi Number:
30061496
Ncbi Gene Id Number:
9001
Background Reference 1:
Takeshita Y, Fujinaga R, Zhao C, Yanai A, Shinoda K. Huntingtin-associated protein 1 (HAP1) interacts with androgen receptor (AR) and suppresses SBMA-mutant-AR-induced apoptosis. Hum Mol Genet. 2006 Aug 1;15(15):2298-312. Epub 2006 Jun 16.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-721

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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