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Western blot analysis of extracts of various cell lines, using HAPLN1 antibody (13-823).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse spleen using HAPLN1 antibody (13-823) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using HAPLN1 antibody (13-823) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using HAPLN1 antibody (13-823) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human spleen using HAPLN1 antibody (13-823) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of BT-20 cells using HAPLN1 antibody (13-823).
Immunofluorescence analysis of HUVEC cells using HAPLN1 antibody (13-823).
Immunofluorescence analysis of SW480 cells using HAPLN1 antibody (13-823).
Western blot analysis of extracts of various cell lines, using HAPLN1 antibody (13-823).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

HAPLN1 Antibody [B10G7]

CATALOG NUMBER: 13-823

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human HAPLN1
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
BT-20
Positive Control 2:
HUVEC
Positive Control 3:
SW480
Predicted Molecular Weight:
Observed: 50kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HAPLN1
Additional Names:
CRT1, CRTL1, hyaluronan and proteoglycan link protein 1, Cartilage link protein, cartilage-link protein, cartilage-linking protein 1, proteoglycan link protein
Ncbi Gene Id Number:
1404
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-823

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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