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IHC testing of human tonsil stained with HCAM antibody (156-3C11).
IHC testing of human breast cancer stained with HCAM antibody (156-3C11).
SDS-PAGE Analysis of Purified, BSA-Free HCAM Antibody (clone 156-3C11). Confirmation of Integrity and Purity of the Antibody.
IHC testing of human tonsil stained with HCAM antibody (156-3C11).

HCAM Antibody [156-3C11]

CATALOG NUMBER: 33-098

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
African Green Monkey, Baboon, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
African Green Monkey, Baboon, Human
Immunogen:
Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, WB
Application Note:
ELISA: order BSA-free format for coating
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCAM antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CD44
Additional Names:
CD44, CD44 molecule (Indian blood group), CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen, GP90 lymphocyte homing/adhesion receptor, Hermes antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglyc
Ncbi Gene Id Number:
960
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody recognizes a cell surface glycoprotein of 80-95kDa, called CD44, or HCAM, on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44-s). Higher molecular weight isoforms are described in epithelial cells (CD44-v), which are believed to function in intercellular adhesion and stromal binding. HCAM antibody immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-098

List Size:
100 ug

List Price:

$626.00

Datasheet
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