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45-724 (0.01ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-724 (0.01ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

hCAP-H Antibody

CATALOG NUMBER: 45-724

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-TEDLSDVLVRQGD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in lysates of cell line K562 (calculated MW of 82.6kDa according to NP_056156.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cabello et al, Mol Biol Cell. 2001 Nov;12(11):3527-37.; PMID: 11694586). Recommended concentration: 0.01-0.03ug/ml.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NCAPH
Additional Names:
HCAP-H, NCAPH, non-SMC condensin I complex, subunit H, BRRN1, CAP-H, XCAP-H homolog, barren homolog 1, chromosome-associated protein H, condensin complex subunit 2
Protein Accession Number:
NP_056156.2
Protein Gi Number:
25121987
Ncbi Gene Id Number:
23397
Background Reference 1:
Torres-Rosell J, De Piccoli G, Cordon-Preciado V, Farmer S, Jarmuz A, Machin F, Pasero P, Lisby M, Haber JE, Aragon L. Anaphase onset before complete DNA replication with intact checkpoint responses. Science. 2007 Mar 9;315(5817):1411-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-724

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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