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This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.5 mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Proteinase K antigen retrieval is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.5 mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Proteinase K antigen retrieval is recommended. Optimal concentrations and conditions may vary.

HCC-1 Antibody

CATALOG NUMBER: XP-5151

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hHCC-1 human Macrophage-Derived Chemokine).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
To detect hHCC-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hHCC-1.
Sandwich: To detect hHCC-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human HCC-1 (XP-5151Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHCC-1.
Western Blot:
To detect hHCC-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHCC-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hHCC-1 specific antibody was purified by affinity chromatography employing immobilized hHCC-1 matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
HCC-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CCL14
Additional Names:
CC-1, CC-3, CKB1, MCIF, NCC2, SY14, HCC-1, HCC-3, NCC-2, SCYL2, SCYA14, HCC-1(1-74), HCC-1/HCC-3, C-C motif chemokine 14,
Protein Accession Number:
Q16627
Protein Gi Number:
2493670
Ncbi Gene Id Number:
6358
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5151

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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