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Antibody used in WB on transformed bacteria at: 1:1000 (Lane1: HIS-HCFC1 16-363aa (42kD) transformed bacteria lysate, Lane2: GFP-HCFC1 363-2002aa (73kD) transformed bacteria lysate elution sample).
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.
Antibody used in WB on transformed bacteria at: 1:1000 (Lane1: HIS-HCFC1 16-363aa (42kD) transformed bacteria lysate, Lane2: GFP-HCFC1 363-2002aa (73kD) transformed bacteria lysate elution sample).

HCFC1 Antibody

CATALOG NUMBER: 28-904

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HCFC1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
HCFC1 antibody can be used for detection of HCFC1 by ELISA at 1:312500. HCFC1 antibody can be used for detection of HCFC1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
45 kDa, 209 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store HCFC1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
HCFC1
Additional Names:
HCFC1, CFF, HCF1, HFC1, MRX3, VCAF, HCF-1
Protein Accession Number:
AAH63435
Protein Gi Number:
39795217
Ncbi Gene Id Number:
3054
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HCFC1 is a member of the host cell factor family with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection.
Background Reference 1:
Director MGC Project, (2003) Direct Submission.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-904

List Size:
100 ul

List Price:

$473.00

Datasheet
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