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Western blot analysis in A549 cell line lysates (35ug/lane).
HCK Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A549 cell line lysates (35ug/lane).

HCK Antibody

CATALOG NUMBER: 63-162

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Monkey
Immunogen:
This HCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-156 amino acids from the N-terminal region of human HCK.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HCK
Additional Names:
Tyrosine-protein kinase HCK, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck, HCK
Protein Accession Number:
P08631
Protein Gi Number:
20141296
Ncbi Gene Id Number:
3055
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HCK is a member of the Srcfamily of tyrosine kinases. This protein is primarily hemopoietic,particularly in cells of the myeloid and B-lymphoid lineages. Itmay help couple the Fc receptor to the activation of therespiratory burst. In addition, it may play a role in neutrophilmigration and in the degranulation of neutrophils. Multipleisoforms with different subcellular distributions are produced dueto both alternative splicing and the use of alternative translationinitiation codons, including a non-AUG (CUG) codon. [provided byRefSeq].
Background Reference 1:
Hassan, R., et al. J. Cell. Physiol. 221(2):458-468(2009)
Background Reference 2:
Kennah, E., et al. Blood 113(19):4646-4655(2009)
Background Reference 3:
Voss, M., et al. BMC Immunol. 10, 53 (2009) :
Background Reference 4:
Rikova, K., et al. Cell 131(6):1190-1203(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-162

List Size:
400 ul

List Price:

$495.00

Datasheet
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