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Western blot analysis of extracts from JurKat cells using HCK (Phospho-Tyr521) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from JurKat cells using HCK (Phospho-Tyr521) Antibody. The lane on the right is treated with the antigen-specific peptide.

HCK (phospho Tyr521) Antibody

CATALOG NUMBER: 79-931

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
HCK (phospho Tyr521) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 521 (E-S-Q (p)-Y-Q) derived from Human HCK .
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous levels of HCK only when phosphorylated at tyrosine 521.
Predicted Molecular Weight:
60 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
HCK
Additional Names:
p59-HCK/p60-HCK, Hemopoietic cell kinas, Tyrosine-protein kinase HCK
Protein Accession Number:
NP_002101.2
Protein Gi Number:
287326139
Ncbi Gene Id Number:
3055
Background:
The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.
Background Reference 1:
Quintrell N., Mol. Cell. Biol. 7:2267-2275(1987) [PubMed: 3496523].
Background Reference 2:
Ziegler S.F.,Mol. Cell. Biol. 7:2276-2285(1987) [PubMed: 3453117].
Background Reference 3:
Ota T., Nat. Genet. 36:40-45(2004) [PubMed: 14702039].

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-931

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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