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Western blot analysis of lysates from K562, Raji cell line (from left to right), using HCLS1 Antibody at 1:1000 at each lane.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
HCLS1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of lysates from K562, Raji cell line (from left to right), using HCLS1 Antibody at 1:1000 at each lane.

HCLS1 Antibody

CATALOG NUMBER: 57-433

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HCLS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 362-391 amino acids from the C-terminal region of human HCLS1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HCLS1
Additional Names:
Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1, LckBP1, p75, HCLS1, HS1
Protein Accession Number:
P14317
Protein Gi Number:
317373440
Ncbi Gene Id Number:
3059
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
Background Reference 1:
Muzio, M., et al. Leukemia 21(9):2067-2070(2007)
Background Reference 2:
Lim, J., et al. Cell 125(4):801-814(2006)
Background Reference 3:
Hao, J.J., et al. J. Biol. Chem. 280(45):37988-37994(2005)
Background Reference 4:
Tao, W.A., et al. Nat. Methods 2(8):591-598(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-433

List Size:
400 ul

List Price:

$495.00

Datasheet
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