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Western blot analysis of various lysates, using HCoV-OC43 Spike S1 Rabbit mAb at 1:8000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.
Western blot analysis of various lysates, using HCoV-OC43 Spike S1 Rabbit mAb at 1:8000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 180s.

HCoV-OC43 Spike S1 Rabbit Recombinant Antibody (82-374)

CATALOG NUMBER: 82-374

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:2000 - 1:9000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Recombinant Human Coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECD His Tag)
Predicted Molecular Weight:
150kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HCoV-OC43 Spike S1
Protein Accession Number:
P36334
Ncbi Gene Id Number:
39105218
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
S1 attaches the virion to the cell membrane by interacting with sialic acid-containing cell receptors, initiating the infection.; [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-374

List Size:
100 uL

List Price:

$477.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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