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Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Jurkat cell line lysates (35ug/lane).
Western blot analysis in mouse kidney tissue lysates (35ug/lane).
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.

HCRTR2 Antibody

CATALOG NUMBER: 58-425

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This HCRTR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-304 amino acids from the Central region of human HCRTR2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
51 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HCRTR2
Additional Names:
Orexin receptor type 2, Ox-2-R, Ox2-R, Ox2R, Hypocretin receptor type 2, HCRTR2
Protein Accession Number:
O43614
Ncbi Gene Id Number:
3062
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a G-protein coupledreceptor involved in the regulation of feeding behavior. Theencoded protein binds the hypothalamic neuropeptides orexin A andorexin B. A related gene (HCRTR1) encodes a G-protein coupledreceptor that selectively binds orexin A.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Pinheiro, A.P., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Background Reference 3:
Chen, J., et al. Biochem. J. 427(3):377-390(2010)
Background Reference 4:
Fellay, J., et al. PLoS Genet.(12), E1000791 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-425

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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