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Western blot analysis of extracts of various cell lines, using HDAC2 antibody (18-488) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using HDAC2 antibody (18-488). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug HDAC2 antibody (18-488). Western blot was performed from the immunoprecipitate using HDAC2 antibody (18-488) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using HDAC2 antibody (18-488) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

HDAC2 Antibody

CATALOG NUMBER: 18-488

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 390 to the C-terminus of human HDAC2 (NP_001518.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IP: 1:50 - 1:200
Positive Control 1:
K-562
Positive Control 2:
NIH/3T3
Positive Control 3:
COS-1
Positive Control 4:
COS-7
Positive Control 5:
HeLa
Positive Control 6:
Jurkat
Predicted Molecular Weight:
Observed: 60kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HDAC2
Additional Names:
HDAC2 Antibody: HD2, RPD3, YAF1, Histone deacetylase 2, HD2
Ncbi Gene Id Number:
3066
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-488

List Size:
100 uL

List Price:

$436.00

Datasheet
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