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Western blot analysis in HL-60 cell line lysates (35ug/lane).
HDAC3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

HDAC3 Antibody

CATALOG NUMBER: 56-975

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This HDAC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-412 amino acids from the C-terminal region of human HDAC3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
49 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HDAC3
Additional Names:
Histone deacetylase 3, HD3, RPD3-2, SMAP45, HDAC3
Protein Accession Number:
O15379
Ncbi Gene Id Number:
8841
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones play a critical role in transcriptionalregulation, cell cycle progression, and developmental events.Histone acetylation/deacetylation alters chromosome structure andaffects transcription factor access to DNA. The protein encoded bythis gene belongs to the histone deacetylase/acuc/apha family. Ithas histone deacetylase activity and represses transcription whentethered to a promoter. It may participate in the regulation oftranscription through its binding with the zinc-fingertranscription factor YY1. This protein can also down-regulate p53function and thus modulate cell growth and apoptosis. This gene isregarded as a potential tumor suppressor gene. [provided byRefSeq].
Background Reference 1:
Minamiya, Y., et al. Tumour Biol. 31(5):533-539(2010)
Background Reference 2:
Kim, H.C., et al. Cell. Mol. Life Sci. 67(20):3499-3510(2010)
Background Reference 3:
Yang, Z., et al. Clin. Chem. Lab. Med. (2010) In press :
Background Reference 4:
Kim, T., et al. Psychiatry Res 178(2):266-269(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-975

List Size:
400 ul

List Price:

$495.00

Datasheet
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