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Western blot analysis of lysed extracts from HepG2 cells using HDAC4 (Ab-632).
Immunofluorescence staining of methanol-fixed HeLa cells using HDAC4 (Ab-632).
Western blot analysis of lysed extracts from HepG2 cells using HDAC4 (Ab-632).

HDAC4 (Ab 632) Antibody

CATALOG NUMBER: 79-557

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
HDAC4 (Ab-632) antibody was raised against a peptide sequence around aa. 630~634 (A-Q-S-S-P) derived from Human HDAC4.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total HDAC4 protein.
Predicted Molecular Weight:
140 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
HDAC4
Additional Names:
HD4, AHO3, BDMR, HDACA, HA6116, HDAC-4, HDAC-A, HD4, Histone deacetylase 4
Protein Accession Number:
NP_006028.2
Protein Gi Number:
153085395
Ncbi Gene Id Number:
9759
Background:
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
Background Reference 1:
Wang AH, et al. (2000) Mol Cell Biol. 20 (18): 6904-6912. Grozinger CM, et al. (2000) Proc Natl Acad Sci U S A. 97 (14): 7835-7840.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-557

List Size:
0.1 mg

List Price:

$468.00

Datasheet
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