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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
HDAC4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Fluorescent confocal image of Hela cell stained with HDAC4 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC4 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HDAC4 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

HDAC4 Antibody

CATALOG NUMBER: 57-218

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HDAC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1049-1077 amino acids from the C-terminal region of human HDAC4.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
119 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HDAC4
Additional Names:
Histone deacetylase 4, HD4, HDAC4, KIAA0288
Protein Accession Number:
P56524
Protein Gi Number:
259016348
Ncbi Gene Id Number:
9759
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones play a critical role in transcriptionalregulation, cell cycle progression, and developmental events.Histone acetylation/deacetylation alters chromosome structure andaffects transcription factor access to DNA. The protein encoded bythis gene belongs to class II of the histone deacetylase/acuc/aphafamily. It possesses histone deacetylase activity and repressestranscription when tethered to a promoter. This protein does notbind DNA directly, but through transcription factors MEF2C andMEF2D. It seems to interact in a multiprotein complex with RbAp48and HDAC3.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Williams, S.R., et al. Am. J. Hum. Genet. 87(2):219-228(2010)
Background Reference 3:
Kim, T., et al. Psychiatry Res 178(2):266-269(2010)
Background Reference 4:
Pan, L., et al. Cell. Mol. Immunol. 7(3):221-226(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-218

List Size:
400 ul

List Price:

$495.00

Datasheet
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