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Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with EGF (lane 2) using HDAC4 (Phospho-Ser632).
Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with EGF (lane 2) using HDAC4 (Phospho-Ser632).

HDAC4 (phospho Ser632) Antibody

CATALOG NUMBER: 79-468

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
HDAC4 (Phospho-Ser632) antibody was raised against a peptide sequence around phosphorylation site of serine 632 (A-Q-S (p) -S-P) derived from Human HDAC4.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of HDAC4 only when phosphorylated at serine 632.
Predicted Molecular Weight:
140 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
HDAC4
Additional Names:
HD4, AHO3, BDMR, HDACA, HA6116, HDAC-4, HDAC-A, AML1, CBFA2, HD4
Protein Accession Number:
NP_006028.2
Protein Gi Number:
153085395
Ncbi Gene Id Number:
9759
Background:
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
Background Reference 1:
Wang AH, et al. (2000) Mol Cell Biol. 20 (18): 6904-6912. Grozinger CM, et al. (2000) Proc Natl Acad Sci U S A. 97 (14): 7835-7840.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-468

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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