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Western blot analysis of extracts of various cell lines, using HDAC6 antibody (13-963) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using HDAC6 antibody (13-963) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug HDAC6 antibody (13-963). Western blot was performed from the immunoprecipitate using HDAC6 antibody (13-963) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using HDAC6 antibody (13-963) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

HDAC6 Antibody

CATALOG NUMBER: 13-963

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 836-1104 of human HDAC6 (NP_006035.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
U-87MG
Positive Control 2:
SKOV3
Positive Control 3:
HepG2
Positive Control 4:
SW620
Positive Control 5:
BT-474
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 170kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HDAC6
Additional Names:
HDAC6 Antibody: HD6, JM21, CPBHM, KIAA0901, Histone deacetylase 6, HD6
Ncbi Gene Id Number:
10013
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-963

List Size:
100 uL

List Price:

$436.00

Datasheet
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