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Western blot analysis of extracts of HeLa cells, using HDAC7 antibody (18-889).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat testis using HDAC7 antibody (18-889) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HDAC7 antibody (18-889) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using HDAC7 antibody (18-889) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of HeLa cells, using HDAC7 antibody (18-889).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

HDAC7 Antibody

CATALOG NUMBER: 18-889

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human HDAC7
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:200
Positive Control 1:
HeLa
Predicted Molecular Weight:
Observed: 65kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HDAC7
Additional Names:
Histone deacetylase 7, HD7, Histone deacetylase 7A, HD7a, HDAC7, HDAC7A
Ncbi Gene Id Number:
51564
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-889

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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