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Western blot analysis in HL-60 cell line lysates (35ug/lane).
HDAC7 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

HDAC7 Antibody

CATALOG NUMBER: 57-176

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HDAC7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 846-875 amino acids from the C-terminal region of human HDAC7.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
103 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HDAC7
Additional Names:
Histone deacetylase 7, HD7, Histone deacetylase 7A, HD7a, HDAC7, HDAC7A
Protein Accession Number:
Q8WUI4
Protein Gi Number:
30913097
Ncbi Gene Id Number:
51564
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones play a critical role in transcriptionalregulation, cell cycle progression, and developmental events.Histone acetylation/deacetylation alters chromosome structure andaffects transcription factor access to DNA. The protein encoded bythis gene has sequence homology to members of the histonedeacetylase family. This gene is orthologous to mouse HDAC7 genewhose protein promotes repression mediated via the transcriptionalcorepressor SMRT. Alternatively spliced transcript variantsencoding different isoforms have been found for this gene.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Margariti, A., et al. Circ. Res. 106(7):1202-1211(2010)
Background Reference 3:
Hutt, D.M., et al. Nat. Chem. Biol. 6(1):25-33(2010)
Background Reference 4:
Malik, S., et al. Mol. Cell. Biol. 30(2):399-412(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-176

List Size:
400 ul

List Price:

$495.00

Datasheet
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