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Western blot analysis of extracts of various cell lines, using HDLBP antibody (19-982) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat liver using HDLBP antibody (19-982) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using HDLBP antibody (19-982) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HDLBP antibody (19-982) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using HDLBP antibody (19-982) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

HDLBP Antibody

CATALOG NUMBER: 19-982

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1035-1268 of human HDLBP (NP_976221.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
Jurkat
Positive Control 3:
HepG2
Positive Control 4:
Mouse brain
Positive Control 5:
NIH/3T3
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 150kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HDLBP
Additional Names:
HDLBP, FLJ16432, HBP, PRO2900, VGL
Ncbi Gene Id Number:
3069
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene binds high density lipoprotein (HDL) and may function to regulate excess cholesterol levels in cells. The encoded protein also binds RNA and can induce heterochromatin formation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-982

List Size:
100 uL

List Price:

$436.00

Datasheet
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