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Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis in A549 cell line lysates (35ug/lane).

HECTD3 Antibody

CATALOG NUMBER: 59-211

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HECTD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 740-769 amino acids from the C-terminal region of human HECTD3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
97 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HECTD3
Additional Names:
E3 ubiquitin-protein ligase HECTD3, 632-, HECT domain-containing protein 3, HECTD3
Protein Accession Number:
Q5T447
Protein Gi Number:
74744877
Ncbi Gene Id Number:
79654
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8 (By similarity).
Background Reference 1:
Yu, J., et al. Biochem. Biophys. Res. Commun. 367(4):805-812(2008)
Background Reference 2:
Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-211

List Size:
400 ul

List Price:

$495.00

Datasheet
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