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Formalin/paraffin human stomach stained with Helicobacter pylori antibody.
Formalin/paraffin human stomach stained with Helicobacter pylori antibody.

Helicobacter Pylori Antibody

CATALOG NUMBER: 33-248

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC
Host Species:
Rabbit
Species Reactivity:
Helicobacter pylori
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Helicobacter pylori
Immunogen:
Total sonicate of Helicobacter ylori was used as the immunogen for this antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Purification:
Protein A affinity chromatography
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Gene Id Number:
NotApplicable
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. This bacteria is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual Helicobacter pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-248

List Size:
100 ug

List Price:

$626.00

Datasheet
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