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Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Hep Par 1 antibody (OCH1E5).
SDS-PAGE Analysis of Purified, BSA-Free Hep Par 1 Antibody (clone OCH1E5). Confirmation of Integrity and Purity of the Antibody.
Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Hep Par 1 antibody (OCH1E5).

Hep Par 1 Antibody [OCH1E5]

CATALOG NUMBER: 33-249

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Dog, Human
Immunogen:
Extract of a formalin-fixed, rejected-allograft of a human liver was used as the immunogen for this Hep Par 1 antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
IF: use antibody at 0.5-1.0 ug/ml
IHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Hep Par 1 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G purified antibody
Clonality:
Monoclonal
Isotype:
IgG1
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Gene Id Number:
Unknown
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or Hep Par 1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC’s occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-249

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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