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Western blot analysis of extracts of various cells, using Heparanase 1 antibody (16-627) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using Heparanase 1 antibody (16-627) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Heparanase 1 antibody (16-627) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cells, using Heparanase 1 antibody (16-627) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Heparanase 1 Antibody

CATALOG NUMBER: 16-627

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human Heparanase 1
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
BxPC-3
Positive Control 3:
A-431
Positive Control 4:
mouse spleen
Positive Control 5:
mouse lung
Positive Control 6:
rat spleen
Predicted Molecular Weight:
Observed: 50kDa/65kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HPSE
Additional Names:
HPSE, Endo-glucoronidase, Heparanase-1, Heparanase, HPA, HSE1, HEP, HPA1, HPSE1, HPR1
Ncbi Gene Id Number:
10855
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-627

List Size:
100 uL

List Price:

$436.00

Datasheet
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