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Western blot analysis of lysed extracts from SKOV3 and MDA cells using HER2 (Ab-1221/1222).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 (Ab-1221/1222).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF cells using HER2 (Ab-1221/1222).
Western blot analysis of lysed extracts from SKOV3 and MDA cells using HER2 (Ab-1221/1222).

HER2 (Ab 1221 / 1222) Antibody

CATALOG NUMBER: 79-308

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
HER2 (Ab-1221/1222) antibody was raised against a peptide sequence around aa.1219~1223/1220~1224 (N-L-Y-Y -W) derived from Human HER2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total HER2 protein.
Predicted Molecular Weight:
185 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
ERBB2
Additional Names:
NEU, NGL, HER2, TKR1, CD340, HER-2, MLN19, HER-2/neu, REX1, ZNF754, C-erbB-2, ErbB2
Protein Accession Number:
NP_001005862.1
Protein Gi Number:
54792098
Ncbi Gene Id Number:
2064
Background:
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.
Background Reference 1:
Marone R, et al. (2004) Nat Cell Biol; 6 (6): 515-22. Ren Z, et al. (2002) J Biol Chem; 277 (41): 38486-93. Ouyang X, et al. (1998) Carcinogenesis; 19 (11): 2013-9. Ouyang X, et al. (1998) Exp Cell Res; 241 (2): 467-75. Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-308

List Size:
0.1 mg

List Price:

$320.00

Out of stock

Datasheet
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