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Western blot analysis of lysed extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1221/Tyr1222).
Immunofluorescence staining of methanol-fixed MCF cells using HER2 (Phospho-Tyr1221/Tyr1222).
Western blot analysis of lysed extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1221/Tyr1222).

HER2 (phospho Tyr1221 / Tyr1222) Antibody

CATALOG NUMBER: 79-442

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
HER2 (Phospho-Tyr1221/Tyr1222) antibody was raised against a peptide sequence around phosphorylation site of tyrosine1221/1222 (N-L-Y (p) -Y (p) -W) derived from Human HER2.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of HER2 only when phosphorylated at tyrosine1221/tyrosine1222.
Predicted Molecular Weight:
185 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
ERBB2
Additional Names:
NEU, NGL, HER2, TKR1, CD340, HER-2, MLN19, HER-2/neu, GP3A, C-erbB-2, ErbB2
Protein Accession Number:
NP_001005862.1
Protein Gi Number:
54792098
Ncbi Gene Id Number:
2064
Background:
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.
Background Reference 1:
Marone R, et al. (2004) Nat Cell Biol; 6 (6): 515-22. Ren Z, et al. (2002) J Biol Chem; 277 (41): 38486-93.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-442

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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