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Western blot analysis of lysed extracts from A431 cells untreated or treated with EGF using Her3/ErbB3 (phospho-Tyr1328).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Her3/ErbB3 (Phospho-Tyr1328).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using Her3/ErbB3 (phospho-Tyr1328).
Western blot analysis of lysed extracts from A431 cells untreated or treated with EGF using Her3/ErbB3 (phospho-Tyr1328).

Her3 / ErbB3 (phospho Tyr1328) Antibody

CATALOG NUMBER: 79-241

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Her3/ErbB3 (phospho-Tyr1328) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1328 (P-D-Y (p) -W-H) derived from Human Her3/ErbB3.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of Her3/ErbB3 only when phosphorylated at tyrosine 1328.
Predicted Molecular Weight:
185 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
ERBB3
Additional Names:
HER3, LCCS2, ErbB-3, c-erbB3, erbB3-S, MDA-BF-1, c-erbB-3, p180-ErbB3, p45-sErbB3, p85-sErbB3, KIAA0288, Tyrosine kinase-type cell surface receptor HER3
Protein Accession Number:
NP_001005915.1
Protein Gi Number:
54792102
Ncbi Gene Id Number:
2065
Background:
Binds and is activated by neuregulins and NTAK.
Background Reference 1:
Holbro, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 8933-8938. Kobayashi, M. et al. (2003) Oncogene 22, 1294-1301. Kim, H. H. et al. (1994) J. Biol. Chem. 269, 24747-24755. Yarden, Y. and Sliwkowski, M. X. (2001) Nature Rev. Mol. Cell. Biol. 2, 127-137.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-241

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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