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Western blot analysis of extracts of various cell lines, using HERC3 antibody (23-661) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human prostate using HERC3 antibody (23-661) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using HERC3 antibody (23-661) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

HERC3 Antibody

CATALOG NUMBER: 23-661

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HERC3 (NP_055421.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
SH-SY5Y
Positive Control 2:
U-87MG
Positive Control 3:
A-431
Positive Control 4:
Mouse lung
Positive Control 5:
Mouse spinal cord
Predicted Molecular Weight:
Observed: 117kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HERC3
Additional Names:
Probable E3 ubiquitin-protein ligase HERC3, 632-, HECT domain and RCC1-like domain-containing protein 3, HERC3, KIAA0032
Ncbi Gene Id Number:
8916
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-661

List Size:
100 uL

List Price:

$436.00

Datasheet
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