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Antibody used in WB on Mouse embryonic stem at 1:800.
Antibody used in WB on Human Ls174T at 1 ug/ml.
Antibody used in IP on Human K562.
Antibody used in IF on Human Ls174T at 50 ug/ml.
Antibody used in WB on Human Raji cells at 1.25 ug/ml.
Antibody used in WB on Mouse embryonic stem at 1:800.

HES1 Antibody

CATALOG NUMBER: 27-568

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat, Zebrafish
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HES1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
HES1 antibody can be used for detection of HES1 by ELISA at 1:62500. HES1 antibody can be used for detection of HES1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Predicted Molecular Weight:
30 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store HES1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
HES1
Additional Names:
HES1, HHL, HRY, HES-1, bHLHb39
Protein Accession Number:
NP_005515
Protein Gi Number:
5031763
Ncbi Gene Id Number:
3280
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Background Reference 1:
Hoebeke, I., et al., (2006) Blood 107 (7), 2879-2881.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-568

List Size:
100 ul

List Price:

$473.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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