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43-464 (1ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-464 (1ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HES4 Antibody

CATALOG NUMBER: 43-464

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RSAAEHRKVGSRP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24kDa band observed in nuclear lysates of cell line HepG2 (calculated MW of 26.1kDa according to NP_001135939.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 (NP_001135939.1) only.
Predicted Molecular Weight:
Approx 24kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HES4
Additional Names:
HES4, hairy and enhancer of split 4 (Drosophila), bHLHb42, bHLH factor Hes4, class B basic helix-loop-helix protein 42, hHES4, transcription factor HES-4
Protein Accession Number:
NP_001135939.1
Protein Gi Number:
10863967
Ncbi Gene Id Number:
57801
Background Reference 1:
El Yakoubi W, Borday C, Hamdache J, Parain K, Tran HT, Vleminckx K, Perron M, Locker M.Hes4 Controls Proliferative Properties of Neural Stem Cells During Retinal Ontogenesis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-464

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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